Journal: STAR Protocols
Article Title: Protocol for mapping murine myeloid bone marrow progenitors and their differentiation into CD103 + cDC1s and CD301b + cDC2s
doi: 10.1016/j.xpro.2026.104508
Figure Lengend Snippet: From mouse preparation to single cell bone marrow cell suspension (A) Fixation of a euthanized C57BL/6 J mouse to the preparation table. (B) Opening of the abdominal wall. (C and D) Proximal to distal cuts in the direction of the extremities. (E–G) Removal of leg-covering skin and fur. (H) Hip dislocation. (I) Left: Isolation of hindlegs. Bottom: Close-up of the incision. Right: Isolated leg. (J) Left: Feet removal by rotating and pulling. Right: Feet removal by cutting at the ankle joint. (K) Reference picture of connected (left) and disconnected (right) tibia and femur after tissue removal and bone cleaning. (L) Opening of femurs at both ends to allow for flushing out bone marrow. (M) Opening of tibias to allow for flushing out bone marrow. (N) Bone marrow isolation. (O) Reference example of femur (left) and tibia (right). Before flushing (below) and after flushing of bone marrow (above). (P) Left: Correct overlay of the cell suspension on top of Lympholyte-M, without mixing. Right: Lymphocytes collected at the interface after gradient centrifugation.
Article Snippet: C57BL/6 J wildtype mice (female, between 8-15 weeks of age) , Charles River , #C57/BL6′′J′′.
Techniques: Single Cell, Suspension, Isolation, Gradient Centrifugation